diff --git a/AutomatedTesting/Gem/PythonTests/editor/EditorScripts/AssetBrowser_TreeNavigation.py b/AutomatedTesting/Gem/PythonTests/editor/EditorScripts/AssetBrowser_TreeNavigation.py
index dc0565d100..0481d872c2 100644
--- a/AutomatedTesting/Gem/PythonTests/editor/EditorScripts/AssetBrowser_TreeNavigation.py
+++ b/AutomatedTesting/Gem/PythonTests/editor/EditorScripts/AssetBrowser_TreeNavigation.py
@@ -66,7 +66,7 @@ class AssetBrowserTreeNavigationTest(EditorTestHelper):
             return collapse_success and expand_success
 
         # This is the hierarchy we are expanding (4 steps inside)
-        self.file_path = ("AutomatedTesting", "Assets", "ImageGradients", "lumberyard_gsi.png")
+        self.file_path = ("AutomatedTesting", "Assets", "ImageGradients", "image_grad_test_gsi.png")
 
         # 1) Open a new level
         self.test_success = self.create_level(
diff --git a/AutomatedTesting/Gem/PythonTests/editor/test_TreeNavigation.py b/AutomatedTesting/Gem/PythonTests/editor/test_TreeNavigation.py
deleted file mode 100755
index a97e4696d5..0000000000
--- a/AutomatedTesting/Gem/PythonTests/editor/test_TreeNavigation.py
+++ /dev/null
@@ -1,61 +0,0 @@
-"""
-All or portions of this file Copyright (c) Amazon.com, Inc. or its affiliates or
-its licensors.
-
-For complete copyright and license terms please see the LICENSE at the root of this
-distribution (the "License"). All use of this software is governed by the License,
-or, if provided, by the license below or the license accompanying this file. Do not
-remove or modify any license notices. This file is distributed on an "AS IS" BASIS,
-WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
-"""
-
-"""
-C13660195: Asset Browser - File Tree Navigation
-"""
-
-import os
-import pytest
-# Bail on the test if ly_test_tools doesn't exist.
-pytest.importorskip('ly_test_tools')
-import ly_test_tools.environment.file_system as file_system
-import automatedtesting_shared.hydra_test_utils as hydra
-
-test_directory = os.path.join(os.path.dirname(__file__), "EditorScripts")
-log_monitor_timeout = 90
-
-
-@pytest.mark.parametrize('project', ['AutomatedTesting'])
-@pytest.mark.parametrize('level', ['tmp_level'])
-@pytest.mark.usefixtures("automatic_process_killer")
-@pytest.mark.parametrize("launcher_platform", ['windows_editor'])
-class TestTreeNavigation(object):
-
-    @pytest.fixture(autouse=True)
-    def setup_teardown(self, request, workspace, project, level):
-        def teardown():
-            file_system.delete([os.path.join(workspace.paths.engine_root(), project, "Levels", level)], True, True)
-
-        request.addfinalizer(teardown)
-
-        file_system.delete([os.path.join(workspace.paths.engine_root(), project, "Levels", level)], True, True)
-
-    @pytest.mark.test_case_id("C13660195")
-    @pytest.mark.SUITE_periodic
-    def test_TreeNavigation_Asset_Browser(self, request, editor, level, launcher_platform):
-        expected_lines = [
-            "Collapse/Expand tests: True",
-            "Asset visibility test: True",
-            "Scrollbar visibility test: True",
-            "TreeNavigation_Asset_Browser:  result=SUCCESS"
-        ]
-
-        hydra.launch_and_validate_results(
-            request,
-            test_directory,
-            editor,
-            "TreeNavigation_Asset_Browser.py",
-            expected_lines,
-            run_python="--runpython",
-            cfg_args=[level],
-            timeout=log_monitor_timeout,
-        )