diff --git a/Gems/Atom/RPI/Code/Include/Atom/RPI.Reflect/Image/StreamingImageAsset.h b/Gems/Atom/RPI/Code/Include/Atom/RPI.Reflect/Image/StreamingImageAsset.h
index 93d0392e38..2e01f99cd9 100644
--- a/Gems/Atom/RPI/Code/Include/Atom/RPI.Reflect/Image/StreamingImageAsset.h
+++ b/Gems/Atom/RPI/Code/Include/Atom/RPI.Reflect/Image/StreamingImageAsset.h
@@ -91,12 +91,15 @@ namespace AZ
             T GetSubImagePixelValue(uint32_t x, uint32_t y, uint32_t componentIndex = 0, uint32_t mip = 0, uint32_t slice = 0);
 
             //! Retrieve a region of image pixel values (float) for specified mip and slice
+            //! NOTE: The topLeft coordinate is inclusive, whereas the bottomRight is exclusive
             void GetSubImagePixelValues(AZStd::pair<uint32_t, uint32_t> topLeft, AZStd::pair<uint32_t, uint32_t> bottomRight, AZStd::span<float> outValues, uint32_t componentIndex = 0, uint32_t mip = 0, uint32_t slice = 0);
 
             //! Retrieve a region of image pixel values (uint) for specified mip and slice
+            //! NOTE: The topLeft coordinate is inclusive, whereas the bottomRight is exclusive
             void GetSubImagePixelValues(AZStd::pair<uint32_t, uint32_t> topLeft, AZStd::pair<uint32_t, uint32_t> bottomRight, AZStd::span<AZ::u32> outValues, uint32_t componentIndex = 0, uint32_t mip = 0, uint32_t slice = 0);
 
             //! Retrieve a region of image pixel values (int) for specified mip and slice
+            //! NOTE: The topLeft coordinate is inclusive, whereas the bottomRight is exclusive
             void GetSubImagePixelValues(AZStd::pair<uint32_t, uint32_t> topLeft, AZStd::pair<uint32_t, uint32_t> bottomRight, AZStd::span<AZ::s32> outValues, uint32_t componentIndex = 0, uint32_t mip = 0, uint32_t slice = 0);
 
             //! Returns streaming image pool asset id of the pool that will be used to create the streaming image.
diff --git a/Gems/Atom/RPI/Code/Source/RPI.Reflect/Image/StreamingImageAsset.cpp b/Gems/Atom/RPI/Code/Source/RPI.Reflect/Image/StreamingImageAsset.cpp
index a3cfc581b6..286b259696 100644
--- a/Gems/Atom/RPI/Code/Source/RPI.Reflect/Image/StreamingImageAsset.cpp
+++ b/Gems/Atom/RPI/Code/Source/RPI.Reflect/Image/StreamingImageAsset.cpp
@@ -315,9 +315,10 @@ namespace AZ
         {
             AZStd::array<T, 1> values = { aznumeric_cast<T>(0) };
 
-            auto position = AZStd::make_pair(x, y);
+            auto topLeft = AZStd::make_pair(x, y);
+            auto bottomRight = AZStd::make_pair(x + 1, y + 1);
             AZStd::span<T> valueSpan(values.begin(), values.size());
-            GetSubImagePixelValues(position, position, valueSpan, componentIndex, mip, slice);
+            GetSubImagePixelValues(topLeft, bottomRight, valueSpan, componentIndex, mip, slice);
 
             return values[0];
         }
@@ -354,9 +355,9 @@ namespace AZ
                 const uint32_t pixelSize = AZ::RHI::GetFormatSize(imageDescriptor.m_format);
 
                 size_t outValuesIndex = 0;
-                for (uint32_t y = topLeft.second; y <= bottomRight.second; ++y)
+                for (uint32_t y = topLeft.second; y < bottomRight.second; ++y)
                 {
-                    for (uint32_t x = topLeft.first; x <= bottomRight.first; ++x)
+                    for (uint32_t x = topLeft.first; x < bottomRight.first; ++x)
                     {
                         size_t imageDataIndex = (y * width + x) * pixelSize;
 
@@ -381,9 +382,9 @@ namespace AZ
                 const uint32_t pixelSize = AZ::RHI::GetFormatSize(imageDescriptor.m_format);
 
                 size_t outValuesIndex = 0;
-                for (uint32_t y = topLeft.second; y <= bottomRight.second; ++y)
+                for (uint32_t y = topLeft.second; y < bottomRight.second; ++y)
                 {
-                    for (uint32_t x = topLeft.first; x <= bottomRight.first; ++x)
+                    for (uint32_t x = topLeft.first; x < bottomRight.first; ++x)
                     {
                         size_t imageDataIndex = (y * width + x) * pixelSize;
 
@@ -408,9 +409,9 @@ namespace AZ
                 const uint32_t pixelSize = AZ::RHI::GetFormatSize(imageDescriptor.m_format);
 
                 size_t outValuesIndex = 0;
-                for (uint32_t y = topLeft.second; y <= bottomRight.second; ++y)
+                for (uint32_t y = topLeft.second; y < bottomRight.second; ++y)
                 {
-                    for (uint32_t x = topLeft.first; x <= bottomRight.first; ++x)
+                    for (uint32_t x = topLeft.first; x < bottomRight.first; ++x)
                     {
                         size_t imageDataIndex = (y * width + x) * pixelSize;
 
diff --git a/Gems/Atom/RPI/Code/Tests/Image/StreamingImageTests.cpp b/Gems/Atom/RPI/Code/Tests/Image/StreamingImageTests.cpp
index 28e5dbd9fe..5efab95818 100644
--- a/Gems/Atom/RPI/Code/Tests/Image/StreamingImageTests.cpp
+++ b/Gems/Atom/RPI/Code/Tests/Image/StreamingImageTests.cpp
@@ -752,7 +752,7 @@ namespace UnitTest
         // Validate retrieving a region of pixels
         AZStd::vector<float> pixelValues(size.m_width * size.m_height);
         auto topLeft = AZStd::make_pair<uint32_t, uint32_t>(0, 0);
-        auto bottomRight = AZStd::make_pair<uint32_t, uint32_t>(size.m_width - 1, size.m_height - 1);
+        auto bottomRight = AZStd::make_pair<uint32_t, uint32_t>(size.m_width, size.m_height);
         streamingImageAsset->GetSubImagePixelValues(topLeft, bottomRight, pixelValues);
         for (uint32_t index = 0; index < pixelValues.size(); ++index)
         {