diff --git a/Code/Framework/AzToolsFramework/AzToolsFramework/Prefab/PrefabFocusHandler.cpp b/Code/Framework/AzToolsFramework/AzToolsFramework/Prefab/PrefabFocusHandler.cpp
index 2338c8892b..4fd202a73d 100644
--- a/Code/Framework/AzToolsFramework/AzToolsFramework/Prefab/PrefabFocusHandler.cpp
+++ b/Code/Framework/AzToolsFramework/AzToolsFramework/Prefab/PrefabFocusHandler.cpp
@@ -83,6 +83,12 @@ namespace AzToolsFramework::Prefab
 
     bool PrefabFocusHandler::IsOwningPrefabBeingFocused(AZ::EntityId entityId)
     {
+        if (!m_focusedInstance.has_value())
+        {
+            // PrefabFocusHandler has not been initialized yet.
+            return false;
+        }
+
         if (entityId == AZ::EntityId())
         {
             return false;