diff --git a/AutomatedTesting/Gem/PythonTests/Multiplayer/CMakeLists.txt b/AutomatedTesting/Gem/PythonTests/Multiplayer/CMakeLists.txt
index 506d15c323..367de4da9a 100644
--- a/AutomatedTesting/Gem/PythonTests/Multiplayer/CMakeLists.txt
+++ b/AutomatedTesting/Gem/PythonTests/Multiplayer/CMakeLists.txt
@@ -7,19 +7,6 @@
 #
 
 if(PAL_TRAIT_BUILD_TESTS_SUPPORTED AND PAL_TRAIT_BUILD_HOST_TOOLS)
-    ly_add_pytest(
-        NAME AutomatedTesting::MultiplayerTests_Main
-        TEST_SUITE main
-        TEST_SERIAL
-        PATH ${CMAKE_CURRENT_LIST_DIR}/TestSuite_Main.py
-        RUNTIME_DEPENDENCIES
-            Legacy::Editor
-            AZ::AssetProcessor
-            AutomatedTesting.Assets
-            AutomatedTesting.ServerLauncher
-        COMPONENT
-            Multiplayer
-    )
     ly_add_pytest(
         NAME AutomatedTesting::MultiplayerTests_Sandbox
         TEST_SUITE sandbox