diff --git a/AutomatedTesting/Gem/PythonTests/largeworlds/CMakeLists.txt b/AutomatedTesting/Gem/PythonTests/largeworlds/CMakeLists.txt
index c2123d683c..98801b49d6 100644
--- a/AutomatedTesting/Gem/PythonTests/largeworlds/CMakeLists.txt
+++ b/AutomatedTesting/Gem/PythonTests/largeworlds/CMakeLists.txt
@@ -24,7 +24,6 @@ if(PAL_TRAIT_BUILD_TESTS_SUPPORTED AND PAL_TRAIT_BUILD_HOST_TOOLS AND PAL_TRAIT_
             LargeWorlds
     )
 
-
     ly_add_pytest(
         NAME AutomatedTesting::DynamicVegetationTests_Periodic
         TEST_SERIAL
@@ -39,6 +38,20 @@ if(PAL_TRAIT_BUILD_TESTS_SUPPORTED AND PAL_TRAIT_BUILD_HOST_TOOLS AND PAL_TRAIT_
             LargeWorlds
     )
 
+    ly_add_pytest(
+        NAME AutomatedTesting::DynamicVegetationTests_Periodic_Optimized
+        TEST_SERIAL
+        TEST_SUITE periodic
+        PATH ${CMAKE_CURRENT_LIST_DIR}/dyn_veg/TestSuite_Periodic_Optimized.py
+        RUNTIME_DEPENDENCIES
+            AZ::AssetProcessor
+            Legacy::Editor
+            AutomatedTesting.Assets
+            AutomatedTesting.GameLauncher
+        COMPONENT
+            LargeWorlds
+    )
+
     ly_add_pytest(
         NAME AutomatedTesting::DynamicVegetationTests_Main_Optimized
         TEST_SERIAL
diff --git a/AutomatedTesting/Gem/PythonTests/largeworlds/dyn_veg/TestSuite_Periodic_Optimized.py b/AutomatedTesting/Gem/PythonTests/largeworlds/dyn_veg/TestSuite_Periodic_Optimized.py
index 764d2dec75..8d298e970b 100644
--- a/AutomatedTesting/Gem/PythonTests/largeworlds/dyn_veg/TestSuite_Periodic_Optimized.py
+++ b/AutomatedTesting/Gem/PythonTests/largeworlds/dyn_veg/TestSuite_Periodic_Optimized.py
@@ -16,7 +16,8 @@ from ly_test_tools.o3de.editor_test import EditorSingleTest, EditorSharedTest, E
 @pytest.mark.parametrize("launcher_platform", ['windows_editor'])
 @pytest.mark.parametrize("project", ["AutomatedTesting"])
 class TestAutomation(EditorTestSuite):
-    global_extra_cmdline_args = []
+
+    global_extra_cmdline_args = ["-BatchMode", "-autotest_mode", "--regset=/Amazon/Preferences/EnablePrefabSystem=true"]
 
     class test_DynVegUtils_TempPrefabCreationWorks(EditorSharedTest):
         from .EditorScripts import DynVegUtils_TempPrefabCreationWorks as test_module