diff --git a/Code/Framework/AzFramework/AzFramework/Scene/Scene.inl b/Code/Framework/AzFramework/AzFramework/Scene/Scene.inl
index b0c1a18b08..360ab93436 100644
--- a/Code/Framework/AzFramework/AzFramework/Scene/Scene.inl
+++ b/Code/Framework/AzFramework/AzFramework/Scene/Scene.inl
@@ -51,7 +51,7 @@ namespace AzFramework
     }
 
     template<typename T>
-    bool Scene::UnsetSubsystem(const T& system)
+    bool Scene::UnsetSubsystem([[maybe_unused]] const T& system)
     {
         const AZ::TypeId& targetType = azrtti_typeid<T>();
         const size_t systemKeysCount = m_systemKeys.size();
diff --git a/Code/Framework/AzToolsFramework/AzToolsFramework/Prefab/PrefabSystemComponent.cpp b/Code/Framework/AzToolsFramework/AzToolsFramework/Prefab/PrefabSystemComponent.cpp
index 3f660bb73b..2570367049 100644
--- a/Code/Framework/AzToolsFramework/AzToolsFramework/Prefab/PrefabSystemComponent.cpp
+++ b/Code/Framework/AzToolsFramework/AzToolsFramework/Prefab/PrefabSystemComponent.cpp
@@ -770,8 +770,8 @@ namespace AzToolsFramework
                 return false;
             }
 
-            Template& sourceTemplate = sourceTemplateReference->get();
 #if defined(AZ_ENABLE_TRACING)
+            Template& sourceTemplate = sourceTemplateReference->get();
             Template& targetTemplate = targetTemplateReference->get();
 #endif
 
diff --git a/Gems/AtomLyIntegration/ImguiAtom/Code/Source/ImguiAtomSystemComponent.cpp b/Gems/AtomLyIntegration/ImguiAtom/Code/Source/ImguiAtomSystemComponent.cpp
index 34e7af688c..8493b78171 100644
--- a/Gems/AtomLyIntegration/ImguiAtom/Code/Source/ImguiAtomSystemComponent.cpp
+++ b/Gems/AtomLyIntegration/ImguiAtom/Code/Source/ImguiAtomSystemComponent.cpp
@@ -99,7 +99,7 @@ namespace AZ
 #endif
         }
 
-        void ImguiAtomSystemComponent::OnViewportSizeChanged(AzFramework::WindowSize size)
+        void ImguiAtomSystemComponent::OnViewportSizeChanged([[maybe_unused]] AzFramework::WindowSize size)
         {
 #if defined(IMGUI_ENABLED)
             ImGui::ImGuiManagerBus::Broadcast([this, size](ImGui::ImGuiManagerBus::Events* imgui)
diff --git a/Gems/LyShine/Code/Source/LyShineDebug.cpp b/Gems/LyShine/Code/Source/LyShineDebug.cpp
index 5ea01c5c1e..76b4030106 100644
--- a/Gems/LyShine/Code/Source/LyShineDebug.cpp
+++ b/Gems/LyShine/Code/Source/LyShineDebug.cpp
@@ -115,11 +115,13 @@ static int Create2DTexture(int width, int height, byte* data, ETEX_Format format
 #endif
 #endif
 
+#if !defined(_RELEASE)
 static AZ::Vector2 GetTextureSize(AZ::Data::Instance<AZ::RPI::Image> image)
 {
     AZ::RHI::Size size = image->GetDescriptor().m_size;
     return AZ::Vector2(size.m_width, size.m_height);
 }
+#endif
 
 ////////////////////////////////////////////////////////////////////////////////////////////////////
 #if !defined(_RELEASE)