diff --git a/Gems/LyShine/Code/Source/Animation/UiAnimationSystem.cpp b/Gems/LyShine/Code/Source/Animation/UiAnimationSystem.cpp
index cab6ae3fa6..ab45042b4c 100644
--- a/Gems/LyShine/Code/Source/Animation/UiAnimationSystem.cpp
+++ b/Gems/LyShine/Code/Source/Animation/UiAnimationSystem.cpp
@@ -37,11 +37,11 @@ namespace
     using UiAnimSystemUnorderedMap = AZStd::unordered_map<KeyType, MappedType, Hasher, EqualKey, AZStd::stateless_allocator>;
 }
 // Serialization for anim nodes & param types
-#define REGISTER_NODE_TYPE(name) assert(g_animNodeEnumToStringMap.contains(eUiAnimNodeType_ ## name)); \
+#define REGISTER_NODE_TYPE(name) assert(!g_animNodeEnumToStringMap.contains(eUiAnimNodeType_ ## name)); \
     g_animNodeEnumToStringMap[eUiAnimNodeType_ ## name] = AZ_STRINGIZE(name);                                                            \
     g_animNodeStringToEnumMap[UiAnimParamSystemString(AZ_STRINGIZE(name))] = eUiAnimNodeType_ ## name;
 
-#define REGISTER_PARAM_TYPE(name) assert(g_animParamEnumToStringMap.contains(eUiAnimParamType_ ## name)); \
+#define REGISTER_PARAM_TYPE(name) assert(!g_animParamEnumToStringMap.contains(eUiAnimParamType_ ## name)); \
     g_animParamEnumToStringMap[eUiAnimParamType_ ## name] = AZ_STRINGIZE(name);                                                              \
     g_animParamStringToEnumMap[UiAnimParamSystemString(AZ_STRINGIZE(name))] = eUiAnimParamType_ ## name;
 
diff --git a/Gems/Maestro/Code/Source/Cinematics/Movie.cpp b/Gems/Maestro/Code/Source/Cinematics/Movie.cpp
index 9c4356560d..2a9a231177 100644
--- a/Gems/Maestro/Code/Source/Cinematics/Movie.cpp
+++ b/Gems/Maestro/Code/Source/Cinematics/Movie.cpp
@@ -87,11 +87,11 @@ namespace
 }
 
 // Serialization for anim nodes & param types
-#define REGISTER_NODE_TYPE(name) assert(g_animNodeEnumToStringMap.contains(AnimNodeType::name)); \
+#define REGISTER_NODE_TYPE(name) assert(!g_animNodeEnumToStringMap.contains(AnimNodeType::name)); \
     g_animNodeEnumToStringMap[AnimNodeType::name] = AZ_STRINGIZE(name);                          \
     g_animNodeStringToEnumMap[AnimParamSystemString(AZ_STRINGIZE(name))] = AnimNodeType::name;
 
-#define REGISTER_PARAM_TYPE(name) assert(g_animParamEnumToStringMap.contains(AnimParamType::name)); \
+#define REGISTER_PARAM_TYPE(name) assert(!g_animParamEnumToStringMap.contains(AnimParamType::name)); \
     g_animParamEnumToStringMap[AnimParamType::name] = AZ_STRINGIZE(name);                           \
     g_animParamStringToEnumMap[AnimParamSystemString(AZ_STRINGIZE(name))] = AnimParamType::name;