diff --git a/AutomatedTesting/Gem/PythonTests/Atom/CMakeLists.txt b/AutomatedTesting/Gem/PythonTests/Atom/CMakeLists.txt
index ff3cd5c465..87a66c880e 100644
--- a/AutomatedTesting/Gem/PythonTests/Atom/CMakeLists.txt
+++ b/AutomatedTesting/Gem/PythonTests/Atom/CMakeLists.txt
@@ -20,19 +20,6 @@ if(PAL_TRAIT_BUILD_HOST_TOOLS AND PAL_TRAIT_BUILD_TESTS_SUPPORTED)
         COMPONENT
             Atom
     )
-    ly_add_pytest(
-        NAME AutomatedTesting::Atom_TestSuite_Main_Optimized
-        TEST_SUITE main
-        PATH ${CMAKE_CURRENT_LIST_DIR}/TestSuite_Main_Optimized.py
-        TEST_SERIAL
-        TIMEOUT 600
-        RUNTIME_DEPENDENCIES
-            AssetProcessor
-            AutomatedTesting.Assets
-            Editor
-        COMPONENT
-            Atom
-    )
     ly_add_pytest(
         NAME AutomatedTesting::Atom_TestSuite_Sandbox
         TEST_SUITE sandbox